Development of rapid, simple and sensitive real-time reverse transcriptase loop-mediated isothermal amplification method (RT-LAMP) to detect viral diseases (PRDV, YHV, IHHNV and TSV) of penaeid shrimp
نویسندگان
چکیده
منابع مشابه
A reverse transcriptase-loop mediated isothermal amplification assay (RT-LAMP) for rapid detection of bovine viral diarrhea virus 1 and 2
Bovine viral diarrhea virus (BVDV) is a pathogen that infects cattle, and is globally important. It causes substantial financial losses to the livestock industry. In the current study, a one-step reverse transcriptase-loop-mediated isothermal amplification (RT-LAMP) assay was set up for rapid and efficient detection of BVDV. For this purpose, four primers were designed to recognize six distinct...
متن کاملThe Penaeid Shrimp Viral Pandemics due to IHHNV, WSSV, TSV and YHV: History in the Americas and Current Status
At least four virus caused pandemics have adversely affected the global penaeid shrimp farming industry since 1980. These viruses in the approximate order of their discovery are Infectious hypodermal and hematopoietic necrosis virus (IHHNV), Yellow Head Virus (YHV), Taura Syndrome Virus (TSV), and White Spot Syndrome Virus (WSSV). The socioeconomic impact of the diseases caused by these viruses...
متن کاملDevelopment of Loop-Mediated Isothermal Amplification (LAMP) Assay for Rapid and Sensitive Identification of Ostrich Meat
Animal species identification is one of the primary duties of official food control. Since ostrich meat is difficult to be differentiated macroscopically from beef, therefore new analytical methods are needed. To enforce labeling regulations for the authentication of ostrich meat, it might be of importance to develop and evaluate a rapid and reliable assay. In the present study, a loop-mediated...
متن کاملDetermination of Herpetic Keratitis Frequency Using Loop Mediated Isothermal Amplification (LAMP) Method in Isfahan, Iran
Herpetic keratitis which is resulted from herpes simplex virus type I, is considered the most common cause of corneal blindness that needs being diagnosed and treated in order to prevent irreversible eye effects. The best method to diagnose it is molecular method which has high sensitivity and exclusivity. The current study was done on 25 eye samples collected from patients referred to the Feyz...
متن کاملDetermination of Herpetic Keratitis Frequency Using Loop Mediated Isothermal Amplification (LAMP) Method in Isfahan, Iran
Herpetic keratitis which is resulted from herpes simplex virus type I, is considered the most common cause of corneal blindness that needs being diagnosed and treated in order to prevent irreversible eye effects. The best method to diagnose it is molecular method which has high sensitivity and exclusivity. The current study was done on 25 eye samples collected from patients referred to the Feyz...
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ژورنال
عنوان ژورنال: Asian Fisheries Science
سال: 2011
ISSN: 0116-6514,2073-3720
DOI: 10.33997/j.afs.2010.23.4.010